Maximum specificity for demanding PCR applications
IBIAN®-HotTaq DNA Polymerase is designed for researchers requiring precise and reproducible results. By using anti-Taq antibodies, the polymerase activity remains inhibited during reaction set-up (20-22 ºC ) , activating exclusively once the temperature exceeds 70 ºC in the thermal cycler.
This feature allows for sample handling at room temperature without the risk of non-specific amplifications, resulting in clean electrophoresis bands and more reliable quantification in RealTime PCR.
Key Features
Exceptional Purity: 98% protein homogeneity verified by 10% SDS-PAGE.
Contaminant-Free: No detectable exo/endonuclease activities.
High Sensitivity: Detection capacity of $\ge6$ DNA molecules.
Buffer Versatility: Includes three buffer variants (NH4 and KCl) to optimize reactions based on target complexity.
Robustness: Effectively directs PCR with templates up to 5 kb in length.
Kit Content (Ref. 101-H02)
IBIAN®-HotTaq DNA Polymerase: 200 units
Storage Conditions
To ensure enzyme stability, the product is shipped on blue-ice. Storage at -20 ºC is recommended. Avoid repeated freeze/thaw cycles