Low EEO para Electroforesis
CAS 9012‐36‐6
Appearance White to off‐white powder
EEO ≤ 0.13
Gelling Point 36°C±1.5°C (1.5% gel)
Melting Point 88°C±1.5°C (1.5% gel)
Solubility Clear colorless solution at 1gr in 100ml water
Moisture ≤ 10%
Gel Strength ≥1200 g/cm2 (1% Gel)
Sulfate ≤ 0.15%
Ash ≤ 0.5%
DNase & RNase None Detected
Protease None Detected
Endonuclease None Detected
Low EEO Agarose Powder is a highly purified agarose suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, producing a cleaner end product with significantly reduced environmental impact. Low EEO Agarose Powder can be used for analysis of proteins and nucleic acids of various sizes (150 bp to 6 kbp).
Its low EEO (≤ 0.13 mr) promotes higher electrophoretic mobility, leading to improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix.
Low EEO Agarose is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion), and various blotting protocols. It is easily soluble, nuclease free and easy to use. It is highly transparent (forms a clear, colorless solution at 1 g:100 mL H2O) and exhibits exceptionally low absorption of chemical coloring agents. The pore size can be adjusted by simple changes in the concentration ratio.
Formulated for high gel strength and integrity, Agarose Powder Low exhibits exceptional thermal stability and mechanical strength, ensuring easy and safe handling, regardless of the addition of a denaturing agent.
Powdered agarose is a medium used in Molecular Biology for electrophoretic separation on an analytical scale in agarose gel electrophoresis, due to its wide separation range and non-toxicity.